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看片视频.CA / Cystic Fibrosis Translational Research Centre (CFTRc) / Platforms / Optical Imaging Facility

Optical Imaging Facility

Objectives Resolution and Sampling

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Theoretical XY Objective Resolution and Nyquist-Shannon Sampling

听 10x/0.3 NA 20x/0.8 NA 40x Water/1.2 NA 63x Oil/1.4 NA
460nm (DAPI) Resolution 935nm 351nm 234nm 200nm
460nm (DAPI) Sampling 407nm 153nm 102nm 87nm
535nm (GFP) Resolution 1088nm 408nm 272nm 233nm
535nm (GFP) Sampling 473nm 177nm 118nm 101nm
620nm (RHOD) Resolution 1261nm 473nm 315nm 270nm
620nm (RHOD) Sampling 548nm 206nm 137nm 117nm
720nm (CY5) Resolution 1464nm 549nm 366nm 314nm
720nm (CY5) Sampling 637nm 239nm 159nm 136nm

Sampling=R/2.3Resolution=0.61*Wavelenght/NA

听

Theoretical Z Objective Resolution听and Nyquist-Shannon Sampling

听 10x/0.3 NA 20x/0.8 NA 40x Water/1.2 NA 63x Oil/1.4 NA
460nm (DAPI) Resolution 5111nm 719nm 319nm 235nm
460nm (DAPI) Sampling 2222nm 313nm 139nm 102nm
535nm (GFP) Resolution 5944nm 836nm 372nm 273nm
535nm (GFP) Sampling 2585nm 363nm 162nm 119nm
620nm (RHOD) Resolution 6889nm 969nm 431nm 316nm
620nm (RHOD) Sampling 2995nm 421nm 187nm 138nm
720nm (CY5) Resolution 8000nm 1125nm 500nm 367nm
720nm (CY5) Sampling 3478nm 489nm 217nm 160nm


Sampling=R/2.3Resolution=Wavelenght/(NA*NA)

Tags:听
CFTRc-Imaging Facility
Objective resolution

Supported by:

听

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听

听

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